EFFECTS OF DIFFERENT APPLICATION PERIODS AND RATES OF MICROBIAL AGENTS ON SOIL MICROBIAL DIVERSITY AND DISEASES OF TOBACCO.

Autor: ZHANG, S. M., CHE, Z. K., WEI, J. Y., ZHANG, J. L., HUANG, C. J., CAI, Y. X., WANG, W.
Předmět:
Zdroj: Applied Ecology & Environmental Research; 2024, Vol. 22 Issue 4, p2885-2902, 18p
Abstrakt: Tobacco (Nicotiana tabacum L.) is a significant cash crop in China. In this study, we investigated the effect of microbial agent application with different rates and application periods on tobacco diseases and soil microorganisms. During the tobacco growing process, the doses of 0, 30 and 60 kg hm-1 were applied at the time of ridging, transplanting and resettling, respectively. 16S rRNA and ITS gene sequencing techniques were utilized to analyze the microbial communities in tobacco fields. The results indicated that the application of microbials significantly decreased the incidence rate and disease index of Ralstonia solanacearum and Phytophthora nicotianae. Moreover, the application of microbial agents resulted in an increase in the abundance of specific fungi (Basidiomycota) and bacteria (Nitrospirae), while reducing the abundance of other fungi (Blastocladiomycota) and bacteria (Spirochaetes). The optimum application dose of microbial agents at the budding stage was recommended as 60 kg.hm-2 for improving tobacco production and soil health, as well as for increasing the economic value of the tobacco leaves of roasted cigarettes significantly. This study emphasizes soil organic matter and pH as key factors influencing tobacco root microbiota. After microbial fertilizer treatment, the relative abundance of various microorganisms in tobacco rhizosphere soil positively correlated with soil enzyme activity and negatively correlated with soil nutrients, which reflected that tobacco rhizosphere microorganisms were regulated by the combination of soil nutrients and enzyme activity. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index