| Autor: |
Crettels, Leslie, Burlion, Nadine, Habets, Audrey, Taminiau, Bernard, Daube, Georges, Delrée, Elisa, Mouchette, Anne-Françoise, Thiry, Damien |
| Předmět: |
|
| Zdroj: |
Journal of Applied Microbiology; Jul2024, Vol. 135 Issue 7, p1-17, 17p |
| Abstrakt: |
Aims The purpose of this work was to study extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBL-EC) in freshwaters, hospital effluents, and wastewaters during two sampling campaigns in 2021. Methods and results Water sampling was performed at 24 stations in the Ourthe watershed in Belgium. A total of 644 ESBL (n = 642) and AmpC (n = 2) E. coli strains were isolated. Disk-diffusion assays were performed following the EUCAST's recommendations. All strains were tested for the presence of bla CTX-M-1, bla CTX-M-2, and bla CTX-M-9 gene groups by PCR. Genes belonging to bla CTX-M-1 and bla CTX-M-9 groups were detected, respectively, in 73.6% and 14.9% of the strains. No bla CTX-M-2 group's gene was found. A subset of strains (n = 40) was selected for whole genome sequencing. Escherichia coli serotype O18: H7 ST 1463 was predominant (n = 14) in the sequenced strains and showed pathogenicity in the Galleria mellonella larvae model. β-lactamase genes identified were bla CTX-M (n = 21), with bla CTX-M-15 mostly represented (n = 15), as well as bla TEM (n = 11), bla OXA (n = 7), bla SHV (n = 9), and carbapenemase (CP) genes were observed in several strains— bla KPC-3 (n = 19), bla NDM-1 (n = 1), bla VIM-1 (n = 2), and bla OXA-244 (n = 2)—even from freshwaters. Conclusions ESBL-EC are widely distributed in the aquatic environment in Belgium and contain a variety of ESBL and CP genes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
