| Abstrakt: |
The Caco-2 cell culture model was envisioned by Dr. Ronald T. Borchardt and established by the brilliant work of Dr. Ismael Hidalgo. As a practitioner of the Caco-2 cell culture model, I witnessed its initial explosive adoption by big pharma and academic research institutions, followed by regulatory agencies, and is now a well-established model. Through the tremendous efforts of many researchers in both industrial and academic organizations, fully characterized and developed Caco-2 cell culture model is now used in absorption screening, phase I and phase II metabolism, efflux transporter study, etc. Major challenges associated with the Caco-2 model include lack of mucous membranes, insufficient cytochrome P450 expression, inadequate expression of many nutrient transporters under standard culture conditions, etc. Despite this significant insufficiency, it is still much better characterized than any other cell culture models of the intestine and will remain an excellent tool for pharmaceutical sciences in decades to come. [ABSTRACT FROM AUTHOR] |
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