| Abstrakt: |
Present study was conducted to determine the optimum concentration of egg yolk in extender for diluting Pantja buck semen and its effect on post-thaw semen quality. Tris-citric acid-fructose-egg yolk-glycerol (TCF-EY) extender containing 4 different levels (5, 10, 15 and 20%) of egg-yolk was used for cryopreservation. Thirty-two (32) ejaculates were collected from four (04) bucks (eight ejaculates from each buck). Semen ejaculates after collection were pooled (4 ejaculates = 1 pooled ejaculate) on daily basis and this pooled ejaculate was divided and diluted into four groups as D1[Neat semen + tris-fructose-citric acid-egg yolk (@5%)-glycerol], D2[Neat semen + tris-fructose-citric acid-egg yolk (@10%)-glycerol], D3[Neat semen + tris-fructose-citric acid-egg yolk (@15%)-glycerol] and D4[Neat semen + tris-fructose-citric acid-egg yolk (@20%)-glycerol], and evaluated for physico-morphological parameters (sperm motility, live spermatozoa count, abnormal spermatozoa percent, plasma membrane integrity and acrosomal integrity) at post-dilution, post-equilibration and post-thaw stages. Biochemical parameters {Malondialdehyde (MDA), Glutamic Oxaloacetic Transaminase (GOT), Glutamic Pyruvate Transaminase (GPT) and Glutathione Peroxidase (GSH-Px)}were also estimated before dilution and at post-thaw stages of semen freezing. Significant difference (P< 0.05) was observed for all the physico-morphological and biochemical parameters at various stage of semen freezing in various diluters. All seminal parameters except total sperm abnormality showed higher values in D2 (10% EY) group at post-dilution, post-equilibration and post-thaw stages compared to other groups. Likewise, significantly lower values for MDA, GOT, GPT and higher value for GSH-Px were observed in D2 group at post-thaw stage of semen freezing. It was therefore, concluded that 10% egg yolk level (V/V) supplementation in Tris-citric acid-fructose-egg yolk -glycerol extender provides comparatively better post-thaw semen quality of Pantja buck semen. [ABSTRACT FROM AUTHOR] |
