| Abstrakt: |
The aim of this study was to determine the production of short chain fatty acids(SCFAs), and antimicrobial activity exhibited by Lactobacillus strains for potential dietary application in-vitro conditions. Probiotic strains can exert positive effects on human health by various mechanisms, among which the production of short chain fatty acids (SCFA). All the SCFA, mainly acetic, propionic and butyric acid, display beneficial effects on human health; SCFA are the most interesting for its role in the prevention and treatment of colonic diseases. Probiotic microbiota plays a vital role in gastrointestinal health and takes other beneficial attributes such as antimicrobial and antibiotic agents. In this study, a total of 10 traditional milk samples were collected from the Southern regions of Tamil Nadu, India. 55 bacterial isolates were isolated based on their sustainability in the gut environment and based on their antagonistic and antioxidant capacity. A series of established in vitro tests were conducted to determine the probiotic potential of isolates. These tests include bile salt tolerance, antibiotic resistance, ability to survive at a lower pH range. From the results 9 bacterial strains showed good antagonistic and antioxidant activity than other strains. Lactobacillus bacteria were identified to genus level by using Lactobacillus specific primers and detected by PCR analysis. Out of these 55 beneficial strains 38 bacterial strains were identified as Lactic acid bacteria and come under the genus Lactobacillus. In this study, the ability of potentially probiotic traditional milk isolated chosen lactic acid bacteria CU31, TG 18, TM 6 to produce SCFA, directly through the production of lactic acid, was investigated. Acetic, lactic, Propionic and butyric acids were quantified by gas chromatography-mass spectrometry (GC-MS) analysis, All the tested strains were confirmed by FTIR analysis. This study suggested that the use of these probiotics may be suitable as an alternative bio prophylactic and bio therapeutic strategy for colon cancer. [ABSTRACT FROM AUTHOR] |
