| Autor: |
De Deus, G., Ximenes, R., Gurgel‐Filho, E. D., Plotkowski, M. C., Coutinho‐Filho, T. |
| Předmět: |
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| Zdroj: |
International Endodontic Journal; Sep2005, Vol. 38 Issue 9, p604-609, 6p |
| Abstrakt: |
Aim To evaluate the cytotoxic effects of two brands of mineral trioxide aggregate (MTA) (Pro-Root MTA® and MTA Angelus®) and Portland cement (PC) on the human ECV 304 endothelial cell line. Methodology Endothelial ECV 304 cells were incubated at 37 °C in an atmosphere of 95% air, 5% carbon dioxide and 100% humidity for 7 days and grown in P12 medium supplemented with 10% fetal bovine serum with 50 μg mL-1 of gentamicin sulphate. Effects of the materials on mitochondrial functions were measured by a colorimetric assay. At each experimental time interval (24, 48 and 72 h), a dimethyl-thiazol-diphenyl tetrazolium bromid assay was conducted to measure cell viability. All assays were repeated three times to ensure reproducibility. Results were expressed as average absorbance (A570\nm) ± SD and the data were analysed statistically by one-way analysis of variance and the Bonferroni post-test. A P-value <0.05 was considered statistically significant. Results No statistically significant difference was shown between any of the experimental materials (P> 0.05). Conclusions The two brands of MTA analysed, as well as the PC, initially showed a similar elevated cytotoxic effect that decreased gradually with time allowing the cell culture to become reestablished. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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