| Autor: |
Hamed, Eman, Al Balah, Osama Fekry Ahmed, Refaat, Mohamed, Badr, Abeer Mahmoud, Afifi, Ahmed |
| Předmět: |
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| Zdroj: |
Lasers in Medical Science; 8/1/2024, Vol. 39 Issue 1, p1-11, 11p |
| Abstrakt: |
Purpose: Conventional approaches for enhancing wound healing may not always yield satisfactory results. Instead, we test the effectiveness of a newly developed photodynamic therapy (PDT) that uses methylene blue (MB) loaded with polyethylene glycol (PEG) (MB-PEG) hydrogel to accelerate wound healing process in mice. Methods: A dorsal skin incision with 6 mm punch which topically subjected to MB-PEG hydrogel and a low-level laser light of red light to assess the regeneration process of wounded skin. A total of 63 adult male CD1 mice divided into normal group (no treatment) and other wound groups received different treatments of laser (650 ± 5 nm and power intensity of 180 mW/cm2), MB-PEG, or PDT (MB-PEG followed by laser). The wound healing parameters were investigated by histological examination of the skin and measuring of proinflammatory cytokines at the early stage (48 h) and a late one on day 21. Results: at 48 h, the score of tissue granulation, inflammation, and angiogenesis process were markedly improved in wounded groups that received MB + PEG combined with laser compared to the group treated with laser alone. On day 21, a significant improvement of the inflammation was detected in the group treated with MB + PEG plus laser compared to the other groups. At 48 h, the upregulated serum levels of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in the wound group were significantly (P < 0.001) reduced in the group treated with MB + PEG combined with laser. Conclusion: MB-PEG based hydrogel improves and accelerates wound closure in the context of laser compared to either single treatment. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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