| Autor: |
Yu-Zhuo Chen, Yi-Hong Zhou, Min-Bo Yan, Ming Xiao, Biao Liu, Ying-Hao Yin, Xiao-Li Tan, Yong-Quan Huang, Yu-Hong Lin, Ting Xie, Jia-Li Tian, Qi Wang, Jian-Ying Li, Zi-Zhou Meng, Zheng Li, Xing, Emily, Yu-Xin Tang, Ya-Wei Li, Zhong-Zhen Su, Liang-Yu Zhao |
| Zdroj: |
Asian Journal of Andrology; May/Jun2024, Vol. 26 Issue 3, p260-267, 9p |
| Abstrakt: |
Ex vivo tissue culture of the human corpus cavernosum (CC) can be used to explore the tissue structural changes and complex signaling networks. At present, artificial CC-like tissues based on acellular or three-dimensional (3D)-printed scaffolds are used to solve the scarcity of primary penis tissue samples. However, inconvenience and high costs limit the wide application of such methods. Here, we describe a simple, fast, and economical method of constructing artificial CC-like tissue. Human CC fibroblasts (FBs), endothelial cells (ECs), and smooth muscle cells (SMCs) were expanded in vitro and mixed with Matrigel in specific proportions. A large number of bubbles were formed in the mixture by vortexing combined with pipette blowing, creating a porous, spongy, and spatial structure. The CC FBs produced a variety of signaling factors, showed multidirectional differentiation potential, and grew in a 3D grid in Matrigel, which is necessary for CC-like tissue to maintain a porous structure as a cell scaffold. Within the CC-like tissue, ECs covered the surface of the lumen, and SMCs were located inside the trabeculae, similar to the structure of the primary CC. Various cell components remained stable for 3 days in vitro, but the EC content decreased on the 7th day. Wingless/integrated (WNT) signaling activation led to lumen atrophy and increased tissue fibrosis in CC-like tissue, inducing the same changes in characteristics as in the primary CC. This study describes a preparation method for human artificial CC-like tissue that may provide an improved experimental platform for exploring the function and structure of the CC and conducting drug screening for erectile dysfunction therapy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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