| Abstrakt: |
Cabbage aphid, Brevicoryne brassicae (L.), is one of the most important pests of cabbage crops. The use of plant-based insecticides, as well as biological control agents such as insect pathogenic fungi, has been considered as complementary methods in integrated pest management. In this research, the effect of Bino2 and two types of entomopathogenic fungi, Metarhizium anisopliae AB and Lecanicillium lecanii 229, as biological insecticides on the protein content, digestive protease activity, chitinase enzyme, acetylcholinesterase and glutathione-S-transferase enzymes of the cabbage aphid adults were examined at a temperature of 25±2 °C, relative humidity of 60±5% and a photoperiod of 16:8 h (L: D). The results indicated that the use of lethal concentration (LC50) of the fungal isolates caused the digestion of protein and chitin in the aphid cuticle, which in turn increased the sensitivity of aphids to these agents by increasing the secretion of protease and chitinase enzymes. Also, the Bino2 insecticide had an anti-nutritional effect by reducing the amount of protein and inhibiting the activity of digestive enzymes. In parallel to the decrease in the activity of digestive enzymes, the activity of detoxifying enzymes also decreased under the influence of the Bino2. The results of this research showed that the tested entomopathogenic fungi and Bino2 insecticide led to an increase in the mortality of cabbage aphids, thus the obtained information can be used in the management of this pest. [ABSTRACT FROM AUTHOR] |
