RT-qPCR investigation of post-mortem tissues during COVID-19.

Autor: Berdygulova, Zhanna, Maltseva, Elina, Perfilyeva, Yuliya, Nizkorodova, Anna, Zhigailov, Andrey, Naizabayeva, Dinara, Ostapchuk, Yekaterina O., Kuatbekova, Saltanat, Dosmagambet, Zhaniya, Kuatbek, Moldir, Bissenbay, Akerke, Cherusheva, Alena, Mashzhan, Akzhigit, Abdolla, Nurshat, Ashimbekov, Sanzhar, Ismagulova, Gulnara, Dmitrovskiy, Andrey, Mamadaliyev, Seidigapbar, Skiba, Yuriy
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Zdroj: Journal of Applied Biomedicine; 2024, Vol. 22 Issue 2, p115-122, 8p
Abstrakt: In 2020, there were numerous cases in Kazakhstan with clinical symptoms of COVID-19 but negative PCR results in nasopharyngeal and oropharyngeal swabs. The diagnosis was confirmed clinically and by CT scans (computed tomography). The problem with such negative PCR results for SARS-CoV-2 infection confirmation still exists and indicates the need to confirm the diagnosis in the bronchoalveolar lavage in such cases. There is also a lack of information about confirmation of SARS-CoV-2 infection in deceased patients. In this study, various tissue materials, including lungs, bronchi, and trachea, were examined from eight patients who died, presumably from SARS-CoV-2 infection, between 2020 and 2022. Naso/oropharyngeal swabs taken from these patients in hospitals tested PCR negative for SARS-CoV-2. This study presents a modified RNA isolation method based on a comparison of the most used methods for RNA isolation in laboratories: QIAamp Viral RNA Mini Kit and TRIzol-based method. This modified nucleic acid extraction protocol can be used to confirm SARS-CoV-2 infection by RT-qPCR in the tissues of deceased patients in disputed cases. RT-qPCR with RNA of SARS-CoV-2 re-extracted with such method from post-mortem tissues that were stored at -80 °C for more than 32 months still demonstrated high-yielding positive results. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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