Rational design of ROS scavenging and fluorescent gold nanoparticles to deliver siRNA to improve plant resistance to Pseudomonas syringae.

Autor: Qi, Jie, Li, Yanhui, Yao, Xue, Li, Guangjing, Xu, Wenying, Chen, Lingling, Xie, Zhouli, Gu, Jiangjiang, Wu, Honghong, Li, Zhaohu
Předmět:
Zdroj: Journal of Nanobiotechnology; 7/29/2024, Vol. 22 Issue 1, p1-17, 17p
Abstrakt: Bacterial diseases are one of the most common issues that result in crop loss worldwide, and the increasing usage of chemical pesticides has caused the occurrence of resistance in pathogenic bacteria and environmental pollution problems. Nanomaterial mediated gene silencing is starting to display powerful efficiency and environmental friendliness for improving plant disease resistance. However, the internalization of nanomaterials and the physiological mechanisms behind nano-improved plant disease resistance are still rarely understood. We engineered the polyethyleneimine (PEI) functionalized gold nanoparticles (PEI-AuNPs) with fluorescent properties and ROS scavenging activity to act as siRNA delivery platforms. Besides the loading, protection, and delivery of nucleic acid molecules in plant mature leaf cells by PEI-AuNPs, its fluorescent property further enables the traceability of the distribution of the loaded nucleic acid molecules in cells. Additionally, the PEI-AuNPs-based RNAi delivery system successfully mediated the silencing of defense-regulated gene AtWRKY1. Compared to control plants, the silenced plants performed better resistance to Pseudomonas syringae, showing a reduced bacterial number, decreased ROS content, increased antioxidant enzyme activities, and improved chlorophyll fluorescence performance. Our results showed the advantages of AuNP-based RNAi technology in improving plant disease resistance, as well as the potential of plant nanobiotechnology to protect agricultural production. Highlights: Polyethyleneimine functionalized gold nanoparticles (PEI-AuNPs) with fluorescent properties and reactive oxygen species (ROS) scavenging ability are synthesized rationally; PEI-AuNPs with fluorescent properties act as the indicator to evaluate the nuclear acid internalization efficiency; PEI-AuNPs platform successfully mediate AtWRKY1 gene silencing and Pst DC3000 resistance by scavenging ROS, increasing antioxidant enzyme activity, and improving photosynthesis. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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