| Autor: |
Kalakonda, Parvathalu, Kathi, Rajitha, Ligory, Merlinsheeba Gali, Dabbeta, Naveenkumar, Madipoju, Naveenkumar, Mynepally, Soujanyalakshmi, Morampudi, Vijay, Banne, Sreenivas, Mandal, Pritam, Savu, Ramu Naidu, Khanam, Sarvani Jowhar, Banavoth, Murali, Sudarsanam Eve, Naina Vinodini, Podila, Bala Bhaskar |
| Zdroj: |
Bioprocess & Biosystems Engineering; Aug2024, Vol. 47 Issue 8, p1307-1319, 13p |
| Abstrakt: |
Our study specifically explores the biosynthesis of copper–silver bimetallic nanoparticles (Cu–Ag BMNPs) using Argyreia nervosa (AN) plant leaf green extract as a versatile agent for capping, reducing, and stabilizing. This biosynthesis method is characterized by its simplicity and cost-effectiveness, utilizing silver nitrate (AgNO3) and cupric oxide (CuO) as precursor materials. Our comprehensive characterization of the Cu–Ag BMNPs, employing techniques such as X-ray diffraction (XRD), UV–Vis spectrometry, scanning electron microscopy (SEM), Zetasizer, and Fourier transformed infrared spectrometry (FTIR). FTIR analysis reveals biofunctional groups and chemical bands, while SEM and XRD analyses provide morphological and structural details. To evaluate the antimicrobial properties of the Cu–Ag BMNPs, we conducted disc diffusion and minimum inhibitory concentration (MIC) assays against Escherichia coli (E. coli), with results compared to the standard gentamicin antibiotic. It is observed that the 2% and 5% CuO concentrations of AN Cu–Ag BMNPs exhibit substantial antibacterial activity in comparison to AN extract when tested on EPEC. Among these, the Cu–Ag BMNPs at a 2% concentration demonstrate higher antibacterial activity, potentially attributed to the enhanced dispersion of BMNPs facilitated by the lower CuO doping concentration. These two assays showcased the improved antimicrobial activity of Cu–Ag BMNPs, highlighting their synergistic effect, characterized by high MIC values and a broad zone of inhibition in the disc diffusion tests against E. coli. These results emphasize the significant antibacterial potential of the synthesized BMNPs, with a medicinal plant AN leaf extract playing a pivotal role in enhancing antibacterial activity. Novelty: The most important aspect of this research project is the role of the Argyreia nervosa plant leaf extract in improving and enhancing the antibacterial activity of the Cu–Ag BMNPs. This underscores the novel synergy between plant extracts and nanomaterials for practical applications. Also, this study's novelty arises from its innovative approach to biosynthesis, the pivotal role of AN plant leaf extract, and the potential for addressing real-world challenges related to bacterial infections and resistance. The enhanced antibacterial properties brought about by this unique synergy have far-reaching implications in diverse applications and underscore the potential of bimetallic nanoparticles in nano-biotechnology. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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