| Autor: |
Aljowaie, Reem M., Alsayed, Mashail Fahad, Alkubaisi, Noorah A., Almarfadi, Omer M., Farrag, Mohamed A., Abdulmanea, Adel A., Alfuraydi, Akram A., Abalkhail, Tarad, Aboul‐Soud, Mourad A. M., Aziz, Ibrahim M. |
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| Zdroj: |
Cell Biochemistry & Function; Jul2024, Vol. 42 Issue 5, p1-16, 16p |
| Abstrakt: |
The main objective of the study is to evaluate the antioxidant, anticancer, and antimicrobial activities of Anchusa officinalis L. in vitro and in silico. The dried aerial parts of A. officinalis L. were extracted with methanol. Total phenolic and flavonoid content was analyzed. Antioxidant and antimicrobial effects were tested against both gram‐positive and gram‐negative bacteria. Gas chromatography‐mass spectrometry analysis revealed the presence of 10 phytochemical compounds, and cyclobutane (26.07%) was identified as the major photochemical compound. The methanol extract exhibited the maximum amount of total phenolic content (118.24 ± 4.42 mg QE/g dry weight of the dry extract) (R2 = 0.994) and the total flavonoid content was 94 ± 2.34 mg QE/g dry weight of the dry extract (R2 = 0.999). The IC50 value for 2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid was 107.12 ± 3.42 μg/mL, and it was high for 1,1‐diphenyl‐2‐picryl hydrazyl (123.94 ± 2.31 μg/mL). The IC50 value was 72.49 ± 3.14 against HepG2 cell lines, and a decreased value was obtained (102.54 ± 4.17 g/mL) against MCF‐7 cell lines. The methanol extract increased the expression of caspase mRNA and Bax mRNA levels when compared to the control experiment (p <.05). The conclusions, A. officinalis L. aerial parts extract exhibited antibacterial, antifungal, and antioxidant activities. Significance statement: The aerial parts of Anchusa officinalis L. were obtained and extracted with methanol.The chemical composition of aerial parts of Anchusa officinalis L. extract and its antioxidant, anticancer, and antibacterial activities were determined.The GC–MS/FID analysis showed that aerial parts of Anchusa officinalis L. extract is an excellent source of curcu hydroquinone and 2,5‐dimethyl‐5‐nitrohexanal.Aerial parts of Anchusa officinalis L. extract preferentially promoted apoptosis in MCF‐7 and HepG2 cells.The findings demonstrated that aerial parts of Anchusa officinalis L. extract have powerful cytotoxic, antibacterial, antifungal, and antioxidant properties, and minimize the risk of developing complex diseases. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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