| Autor: |
Ousalem, Farès, Ngo, Saravuth, Oïffer, Thomas, Omairi-Nasser, Amin, Hamon, Marion, Monlezun, Laura, Boël, Grégory |
| Předmět: |
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| Zdroj: |
Nature Communications; 7/26/2024, Vol. 15 Issue 1, p1-16, 16p |
| Abstrakt: |
Having multiple rounds of translation of the same mRNA creates dynamic complexities along with opportunities for regulation related to ribosome pausing and stalling at specific sequences. Yet, mechanisms controlling these critical processes and the principles guiding their evolution remain poorly understood. Through genetic, genomic, physiological, and biochemical approaches, we demonstrate that regulating ribosome pausing at specific amino acid sequences can produce ~2-fold changes in protein expression levels which strongly influence cell growth and therefore evolutionary fitness. We demonstrate, both in vivo and in vitro, that the ABC-F protein EttA directly controls the translation of mRNAs coding for a subset of enzymes in the tricarboxylic acid (TCA) cycle and its glyoxylate shunt, which modulates growth in some chemical environments. EttA also modulates expression of specific proteins involved in metabolically related physiological and stress-response pathways. These regulatory activities are mediated by EttA rescuing ribosomes paused at specific patterns of negatively charged residues within the first 30 amino acids of nascent proteins. We thus establish a unique global regulatory paradigm based on sequence-specific modulation of translational pausing. The physiological function of the ABC-F translation factor has remained elusive. Here, the authors show that one of them, EttA, regulates the translation of mRNAs encoding early acidic residues, and that impacts the physiology of central metabolism. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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