| Abstrakt: |
An accurate direct DPP identification of ceftazidime has been made for use in drug discovery and validation. The results showed that a mercury drop of 3 mm³, an acidic medium consisting of a KH phthalate plus HCl buffer solution at pH 2, and 1 ml of NH4Cl, 1 M as a supporting electrolyte were optimal for ceftazidime analysis. Ceftazidime exhibited two peaks, the first at 0.67V and the second at 1.06V. For the determination of an unknown concentration, a standard graph of ceftazidime in concentrations ranging from 8.4×10-6 M to 2.6×10-5 M was developed. The reliability and accuracy of this technique for analysing the analyte were assessed. For ceftazidime laboratory samples, the SD was 0.1885 and the RSD% did not go above 4.573% at a concentration of 4.6 µg.m-1, 8.4×10-6 M, and at a concentration of 8.5 µg.ml, 1.5×10-5 M, the SD was 0.35645 and the RSD% did not go over 4.345%. The peak potential Ep, and the actual number of electrons required for the reduction of ceftazidime are both calculated using the Ilkovic-Heyrovsky equation. The data show that ceftazidime requires five electrons for the reduction reaction to occur. The method has been successfully used to get ceftazidime on the market [ABSTRACT FROM AUTHOR] |
