| Abstrakt: |
Operation of radio-genetic 'Inherited sterility technique' (IS), proposed for suppression of a serious tropical pest, Spodoptera litura (Fabr.) requires reproductively competitive moths as a prerequisite. Insects harbor microbes, which might be associated with their reproductive viability. The present study was attempted to assess the gut bacterial diversity of the F1 sixth instar (L6) progeny derived from irradiated (100Gy, 130Gy), sub-sterilized male parent moths in comparison with control, using Next Generation Sequencing (NGS) analysis of 16 S rRNA of the bacterial community of larval gut. Irradiated S.litura showed an increased bacterial load than control. Irradiation could stimulate the abundance of some groups of bacteria in the gut, whereas overall species richness and evenness were affected by irradiation. Phylum Firmicutes was the most abundant phylum in the irradiated larvae (both in the 100Gy F1L6 and 130Gy F1L6); whereas in control larval gut, the Proteobacteria, Bacteroidetes, Chlorofexi, and Cyanobacteria were found to be abundant as compared to the irradiated regimens. Lactobacillus, Bacillus, Paenibacillus, the genera belonging to the phylum Firmicutes were relatively more abundant in the irradiated larvae (100Gy F1L6 and 130Gy F1L6), whereas genera Pseudomonas, Neisseria, and Stenotrophomonas belonging to the phylum Proteobacteria were more abundant in control. The functional pathways involved in the oxidation and reduction of sulfate and nitrate were found to be enriched in the control larval gut whereas the streptomycin production, chloramphenicol degradation pathways were enriched in the irradiated larval gut. Irradiation could significantly alter the structure of S. litura bacteriome, which might be correlated with the degree of inherited sterility in this nuclear tactic, IS. [ABSTRACT FROM AUTHOR] |
Full text from SpringerLink