Analysis of Clinical Samples of Pancreatic Cyst's Lesions with A Multi‐Analyte Bioelectronic Simot Array Benchmarked Against Ultrasensitive Chemiluminescent Immunoassay.

Autor: Scandurra, Cecilia, Björkström, Kim, Caputo, Mariapia, Sarcina, Lucia, Genco, Enrico, Modena, Francesco, Viola, Fabrizio Antonio, Brunetti, Celestino, Kovács‐Vajna, Zsolt M., Franco, Cinzia Di, Haeberle, Lena, Larizza, Piero, Mancini, Maria Teresa, Österbacka, Ronald, Reeves, William, Scamarcio, Gaetano, Wheeler, May, Caironi, Mario, Cantatore, Eugenio, Torricelli, Fabrizio
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Zdroj: Advanced Science; 7/17/2024, Vol. 11 Issue 27, p1-14, 14p
Abstrakt: Pancreatic cancer, ranking as the third factor in cancer‐related deaths, necessitates enhanced diagnostic measures through early detection. In response, SiMoT‐Single‐molecule with a large Transistor multiplexing array, achieving a Technology Readiness Level of 5, is proposed for a timely identification of pancreatic cancer precursor cysts and is benchmarked against the commercially available chemiluminescent immunoassay SIMOA (Single molecule array) SP‐X System. A cohort of 39 samples, comprising 33 cyst fluids and 6 blood plasma specimens, undergoes detailed examination with both technologies. The SiMoT array targets oncoproteins MUC1 and CD55, and oncogene KRAS, while the SIMOA SP‐X planar technology exclusively focuses on MUC1 and CD55. Employing Principal Component Analysis (PCA) for multivariate data processing, the SiMoT array demonstrates effective discrimination of malignant/pre‐invasive high‐grade or potentially malignant low‐grade pancreatic cysts from benign non‐mucinous cysts. Conversely, PCA analysis applied to SIMOA assay reveals less effective differentiation ability among the three cyst classes. Notably, SiMoT unique capability of concurrently analyzing protein and genetic markers with the threshold of one single molecule in 0.1 mL positions it as a comprehensive and reliable diagnostic tool. The electronic response generated by the SiMoT array facilitates direct digital data communication, suggesting potential applications in the development of field‐deployable liquid biopsy. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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