| Abstrakt: |
The objective of this study was to investigate the changes in argyrophilic nucleolar organizer region proteins (Ag-NORs) of pediatric epilepsy and T lymphocyte subpopulation and their relationship before and after treatment, 156 children with epilepsy diagnosed at Yan’an University Affiliated Hospital were selected as the research objects. In addition, 100 healthy children were selected and divided into control group. Flow cytometry was utilized to detect T lymphocyte subpopulation. Common silver staining technique was adopted to detect Ag-NORs of T lymphocyte. There were 85 patients in big seizure group (54.48%) and 41 patients in minor vitality loss seizure group, including 12 with infantile spasm (7.69%), 15 with limited motor seizure (9.61%), and 14 with psychomotor seizure (8.97%). Compared with normal control group, there were 46 children with vegetative nervous seizure (29.48%). The expressions of CD3+, CD4+, CD8+ lymphocyte in epileptic children group, primary epilepsy group, secondary epilepsy group, big seizure group, minor vitality loss seizure group, and vegetative nervous seizure group decreased, while that of CD20+ lymphocyte improved (P<0.05). Compared with that of normal group, Ag-NORs of epileptic children group, primary epilepsy group, secondary epilepsy group, big seizure group, minor vitality loss seizure group, and vegetative nervous seizure group remarkably reduced (P<0.05). Ag-NORs content of T lymphocyte among children with epilepsy was lower than that among normal children. It was concluded that T lymphocyte subpopulation could be replaced by the measurement of Ag-NORs content of T lymphocyte to assess the immune function and status of children with epilepsy. [ABSTRACT FROM AUTHOR] |
