Maximization of Micropropagule Production in an Attractive Ornamental Plant, Oxalis triangularis A.st.-Hil.

Autor: Klaocheed, Sutha, Rittirat, Suphat, Kalawong, Soontreeya, Thammasiri, Kanchit
Předmět:
Zdroj: Trends in Sciences; Aug2024, Vol. 21 Issue 8, p1-9, 9p
Abstrakt: Petiole segments of an economically ornamental plant Oxalis triangularis A.st.-Hil have been used in the present study for rapid, reliable and systematic in vitro propagation. The morphogenic effect of a-Naphthalene acetic acid (NAA) at various concentrations (0.5 - 3.0 mg/L) was studied individually or in combination with 6-benzylaminopurine (BAP). Superior multiplication rates were achieved on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BAP. Of the tested combinations, maximum shoot regeneration (100.00 %), mean shoot number (14.60 ± 0.37 shoots per segment), and shoot length (18.11 ± 0.30 mm) were recorded on MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BAP after 6 weeks of incubation. Half-strength MS medium augmented with 1.0 mg/L indole-3-butyric acid (IBA) was the optimal medium for in vitro root induction, (24.60 ± 0.37 roots per shoot with 9.10 ± 0.32 mm in length) after 6 weeks of incubation. The regenerated plantlets showed 100 % survival success during acclimatization and exhibited normal growth characteristics and morphology. This optimized protocol is cost-effective and highly efficient for the rapid mass propagation of Oxalis triangularis A.st.-Hil. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index