| Autor: |
Paulo, Eloiza Aparecida, de Souza, Cássia Milena, de Souza, Nathália Aparecida Andrade, Quirino, Juliana Nunes, Furlaneto-Maia, Luciana, Furlaneto, Marcia Cristina |
| Zdroj: |
BioNanoScience; Jun2024, Vol. 14 Issue 2, p892-902, 11p |
| Abstrakt: |
Metallic nanoparticles with antimicrobial properties have obtained the status of a new generation of antifungal drugs. This study described a method for the synthesis of biogenic silver nanoparticles (Bio-AgNPs) using cell-free supernatant containing enterocin from Enterococcus durans and assessed the antifungal potential against fluconazole-resistant Candida tropicalis. Bio-AgNPs was confirmed visually by the appearance of a reddish-brown color formation. A sharp absorbance peak at 448 nm was found using a UV-visible spectrophotometer, confirming the presence of Bio-AgNPs. Fourier transform infrared spectroscopy, differential light scattering, energy dispersive spectroscopy, and transmission electronic microscopy analyses were employed to characterize the Bio-AgNP. Reduction of Ag+ ions in solution with CFS from E. durans produced spherical Bio-AgNPs with an average size of 169.11 nm and zeta potential of − 25.3 mV. The anti-Candida activity of Bio-AgNPs and its synergism with fluconazole were determined using agar well diffusion and broth microdilution method. Bio-AgNPs at a concentration of 32.17 μg/mL promoted zones of inhibition that ranged from 18.5 ± 0.7 to 23 ± 1.7 mm among C. tropicalis strains. The minimum inhibitory concentration of Bio-AgNPs that inhibit 80% of growth ranged from 0.251 to 2.01 μg/mL. Bio-AgNPs and fluconazole showed synergistic interaction in 4 out of 6 strains (FICI range 0.23–0.31). These results demonstrate a sustainable and cost-effective method for synthesis of Bio-AgNPs with activity against C. tropicalis. Due to significant activity against fluconazole-resistant strains of C. tropicalis, Bio-AgNPs alone and in combination with fluconazole may represent an alternative in the control of infections associated with this pathogen. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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