| Autor: |
Varma, Manoj M, Badrinath, Shree Sumanas |
| Zdroj: |
BioNanoScience; Jun2024, Vol. 14 Issue 2, p1767-1779, 13p |
| Abstrakt: |
Nucleic acid aptamers are being used extensively for real-time molecular recognition. Aptamers are nucleotide strands that bind to highly specific targets. They also hold distinct features that make them superior to antibodies, such as better stability and longer shelf life. Numerous analytical methods utilize the binding affinity of aptamers to various targets for molecular detection. This paper reviews the need to evaluate the aptasensor's performance and the power optimization of different operating conditions. We also summarize the trend in aptamer-based sensing for molecular diagnostics. As the sensing targets described in the literature are numerous, we pick thrombin as an illustrative target. We focus on the different design parameters involved in developing an aptasensor strategy and the evaluation of the aptasensor's limit of detection (LOD), specificities, reproducibility, and stability. These insights will be beneficial in developing assays where limitations on clinical diagnostics are involved. This review will be helpful to researchers interested in exploring aptamer-based methods for various end applications. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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