| Autor: |
Xingyu Wu, Tingting Zhang, Ke Zhang, Rui Zhang, Man Shi, Chenlei Gu, Tianqiong Shi, Ling Lu, Feng Xue, Qing Xu, Chi Zhang |
| Předmět: |
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| Zdroj: |
Synthetic & Systems Biotechnology; Jun2024, Vol. 9 Issue 2, p277-284, 8p |
| Abstrakt: |
Aspergillus niger is an efficient cell factory for organic acids production, particularly L-malic acid, through genetic manipulation. However, the traditional method of collecting A. niger spores for inoculation is labor-intensive and resource-consuming. In our study, we used the CRISPR-Cas9 system to replace the promoter of brlA, a key gene in Aspergillus conidiation, with a xylose-inducible promoter xylP in L-malic acid-producing A. niger strain RG0095, generating strain brlAxylP. When induced with xylose in submerged liquid culture, brlAxylP exhibited significant upregulation of conidiation-related genes. This induction allowed us to easily collect an abundance of brlAxylP spores (>7.1 × 106/mL) in liquid xylose medium. Significantly, the submerged conidiation approach preserves the substantial potential of A. niger as a foundational cellular platform for the biosynthesis of organic acids, including but not limited to L-malic acid. In summary, our study offers a simplified submerged conidiation strategy to streamline the preparation stage and reduce labor and material costs for industrial organic acid production using Aspergillus species. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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