| Autor: |
Dieu Thu Nguyen, Kokáš, Filip Zavadil, Gonin, Mathieu, Lavarenne, Jérémy, Colin, Myriam, Gantet, Pascal, Bergougnoux, Véronique |
| Zdroj: |
BMC Plant Biology; 5/22/2024, Vol. 24, p1-14, 14p, 2 Charts, 5 Graphs |
| Abstrakt: |
Background Roots play an important role during plant growth and development, ensuring water and nutrient uptake. Understanding the mechanisms regulating their initiation and development opens doors towards root system architecture engineering. Results Here, we investigated by RNA-seq analysis the changes in gene expression in the barley stem base of 1 day-after-germination (DAG) and 10DAG seedlings when crown roots are formed. We identifed 2,333 genes whose expression was lower in the stem base of 10DAG seedlings compared to 1DAG seedlings. Those genes were mostly related to basal cellular activity such as cell cycle organization, protein biosynthesis, chromatin organization, cytoskeleton organization or nucleotide metabolism. In opposite, 2,932 genes showed up-regulation in the stem base of 10DAG seedlings compared to 1DAG seedlings, and their function was related to phytohormone action, solute transport, redox homeostasis, protein modifcation, secondary metabolism. Our results highlighted genes that are likely involved in the diferent steps of crown root formation from initiation to primordia diferentiation and emergence, and revealed the activation of diferent hormonal pathways during this process. Conclusions This whole transcriptomic study is the frst study aiming at understanding the molecular mechanisms controlling crown root development in barley. The results shed light on crown root emergence that is likely associated with a strong cell wall modifcation, death of the cells covering the crown root primordium, and the production of defense molecules that might prevent pathogen infection at the site of root emergence. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
