| Abstrakt: |
Introduction: Sicklepod [Cassia obtusifolia L. syn Senna obtusifolia (L.) H.S. Irwin & Barneby, Fabaceae] sprouts are promising ingredients with health‐promoting benefits. Notwithstanding, the pharmacologically active compounds in sicklepod sprouts have not been studied or analysed in detail. Objective: This study aimed to isolate and structurally identify phytochemicals showing α‐glucosidase inhibitory activity in sicklepod sprouts and simultaneously quantify the compounds in the sprouts to determine the optimal cultivation method and germination time to maximise active compounds. Method: A simultaneous high‐performance liquid chromatography‐ultraviolet (HPLC‐UV) method with high sensitivity and accuracy was developed and used to analyse time‐dependent changes in anthraquinone content during sicklepod germination. Results: Thirteen anthraquinones were isolated and identified, of which six—chrysoobtusin, emodin, 1‐O‐methyl‐2‐methoxychrysophanol, 7‐O‐methylobtusin, chrysophanol, and physcion—showed moderate α‐glucosidase inhibitory activity. The maximum content of anthraquinones in a sprout was observed on Day 5 under both light and dark conditions. Conclusion: The findings of this study revealed that sicklepod sprouts which are promising functional food materials contain a variety of anthraquinones. In this study, the functional characterization and simultaneous HPLC analysis of 13 anthraquinones in sicklepod (Cassia obtusifolia L.) sprouts. The phytochemical investigation revealed that sicklepod sprouts produced various free anthraquinones, which exhibited α‐glucosidase inhibitory activity. Interestingly, the anthraquinone content increased during sprouting. Hypothetically, because hydrolase in the seeds was activated during sprouting and anthraquinone glycosides were changed to free anthraquinones with α‐glucosidase inhibitory activity, the activity of the sprouts was stronger than that of the seeds. [ABSTRACT FROM AUTHOR] |
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