Determination of ertapenem in plasma and ascitic fluid by UHPLC-MS/MS in cirrhotic patients with spontaneous bacterial peritonitis.

Autor:	Rigo-Bonnin, Raúl, Amador, Alberto, Núñez-Gárate, María, Mas-Bosch, Virgínia, Padullés, Ariadna, Cobo-Sacristán, Sara, Castellote, José
Předmět:	CIRRHOSIS of the liver PERITONITIS LIQUID chromatography-mass spectrometry HYPERLIPIDEMIA CHEMICAL reagents ERTAPENEM DESCRIPTIVE statistics STAPHYLOCOCCUS aureus ENTEROBACTERIACEAE ESCHERICHIA coli KLEBSIELLA infections WATER ELECTROSPRAY ionization mass spectrometry BACTERIAL contamination BLOOD plasma BACTERIAL diseases BODY fluids HEMOLYSIS & hemolysins DATA analysis software CALIBRATION SENSITIVITY & specificity (Statistics) TIME PHARMACODYNAMICS
Zdroj:	Advances in Laboratory Medicine / Avances en Medicina de Laboratorio; Jun2024, Vol. 5 Issue 2, p173-180, 8p
Abstrakt:	Spontaneous bacterial peritonitis is a frequent severe complication in cirrhotic patients with ascites. Carbapenem antibiotics are currently the treatment of choice for patients with hospital-acquired or healthcare-related infections. However, there is limited evidence available on the efficacy of ertapenem in cirrhotic patients with spontaneous bacterial peritonitis. As a result, the pharmacokynetics and pharmacodynamics of this antibiotic are still unknown. The objective of this study was to develop and validate measurement procedures based on liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to determine ertapenem concentrations in plasma and ascitic fluid. Samples were pretreated by acetronile protein-precipitation. Chromatographic separation is performed on a C18 reversed-phase Acquity®-UPLC®-BEHTM column (2.1 × 100 mm id, 1.7 µm) using a non-linear gradient of water/acetonitrile containing 0.1 % of formic acid at a flow rate of 0.4 mL/min. Ertapenem and its internal standard (ertapenem-D4) are detected by tandem mass spectrometry using positive electrospray ionization and multiple reaction monitoring, and using 476.2 → 346.0/432.2 as mass transition for ertapenem and 480.2 → 350.0 for its internal standard. No significant interferences or carry-over contamination were observed. Imprecisions, absolute relative bias, matrix effects and normalized recoveries were ≤14.5 %, ≤9.3 % (92.8–104.5) % and (98.8–105.8) %, respectively. Chromatographic measurement procedures were linear from (0.50–100) mg/L. The measurement procedures based on UHPLC-MS/MS developed and validated in this study could be useful in pharmacokynetic and pharmacodynamic studies in subjects with liver cirrhosis who develop spontaneous bacterial peritonitis treated with ertapenem. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu