| Autor: |
Tuteja, Renu, Agarwal, Anshu, Vijayakrishnan, Lalitha, Nayak, Bisho P., Gupta, Satish K., Kumar, Vijay, Rao, Kanury V. S. |
| Předmět: |
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| Zdroj: |
Immunology & Cell Biology; Jun1997, Vol. 75 Issue 3, p245-252, 8p |
| Abstrakt: |
This report analyses murine primary humoral recognition of a linear domain (MEP 17-31) within a 100 amino acid polypeptide, MEP-1. An analysis of the early primary IgM response revealed that MEP 17-31 presented at least two distinct domains for pre-immune B cell recognition represented by MEP-1 residues 19-23 and 26-28. However, subsequent maturation into an IgG response saw an exclusive selection for the anti-MEP 19-23 component with loss of all alternate specificities. The IgM response to MEP 19-23 was oligoclonal and composed of diverse paratope phenotypes as evidenced by varied heavy chains of immunoglobulin V-D-J combinations and CDR3 sequences. In contrast to the oligoclonality of IgM mAb. the mature IgG response to MEP 19-23 appeared to derive predominantly from a single progenitor. It therefore appears that maturation of primary humoral responses to polypeptide antigens involves two distinct levels of selection. While there is selection for a restricted subset of the initially induced antibody fine-specificities, progression of the response also entails a reduction in clonal heterogeneity of B cells responding to the dominant epitope. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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