EVALUATION OF ECO-EXTRACTION METHODS OF ANTIOXIDANTS AND THEIR ACTIVITIES FROM RETAMA RAETAM TWIGS.

Autor: Zaoui, Oussama, Oughlissi-Dehak, Karima, Bouziane, Mebarka, Zaoui, Fatiha, Boudou, Farouk, Benras, Amina, Sehmi, Abdelghani, Hadj-Mahammed, Mahfoud
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Zdroj: Polish Journal of Natural Sciences; 2023, Vol. 38 Issue 4, p309-322, 14p
Abstrakt: The purpose of this study is to compare the effectiveness of different conventional and nonconventional methods for the extraction of secondary metabolites and antioxidant activity of aqueous and hydro-ethanolic extract of Retama raetam twigs including maceration, reflux, Soxhlet, microwave assisted extraction (MAE) and ultrasonic assisted extraction. The aqueous and hydro-ethanolic extracts obtained by MAE showed the highest contents of total phenolics (160.43 ±1.42 and 175.71 ±2.09 mg EAG/g DR, respectively) and flavonoids of 12.28 ±0.92 and 39.97 ±1.11 mg EC/g DR, respectively. It also exhibited significant DPPH• scavenging capacity with IC50 values of 0.45 ±0.075 and 0.34 ±0.039 mg/mL and significant iron reducing capacity with EC50 of 0.358 ±0.02 and 0.28 ±0.01 mg/mL for the aqueous and hydro-ethanolic extracts, respectively. The MAE proved to be the most efficient extraction technique for the extraction of antioxidants from R. raetam twigs. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index