| Abstrakt: |
This study aimed to determine the optimal combination of three anti-infammatory materials [i.e., Cervus nippon Temminck (CT), Angelica gigas Nakai (AN), and Rehmannia glutinosa (RG)] for the strongest anti-infammatory potential. Eighteen combinations of the three materials were tested in LPS-stimulated RAW264.7 cells via assessing nitric oxide (NO). The best combination from in vitro studies was administered to LPS-treated C57BL/6J mice for fve days. Subsequently, plasma metabolites were profled by bioinformatics analyses and validations. As results, 2, 20, and 50 µg/mL of CT, AN, and RG (TM) were the most efective combination suppressing infammation. In mice, TM mitigated hepatic infammatory markers. Similarly, the metabolomics indicated that TM may suppress NF-κB signaling pathway, thereby alleviating hepatic infammation. TM also decreased systemic and hepatic pro-infammatory cytokines. Collectively, we found the optimal combination of TM for mitigating infammation; thus further studies on safety, mechanisms, and clinical models are warranted for human applications. [ABSTRACT FROM AUTHOR] |
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