| Abstrakt: |
The experiment was to study the effects of dietary isochlorogenic acid (ICGA) on early growth performance, intestinal structure, immune performance, and microbial diversity of broilers. 400 one-day-old Kebao broilers, half in male and half female, were randomly divided into five groups with eight replicates in each group and 10 chickens in each replicate. Each group was fed with basal diet (control group), basal diet + 500 mg/kg ICGA (T1 group), basal diet + 1 000 mg/kg ICGA (T2 group), basal diet + 2 000 mg/kg ICGA (T3 group), and basal diet + 3 000 mg/kg ICGA (T4 group). The trial period was 21 days. The results showed that the average daily feed intake (ADFI), average daily gain (ADG), and feed-to-weight ratio (F/G) were not significantly different among the groups (P>0.05). The apparent metabolic rate of crude protein (CP) in T3 group and T4 group was significantly higher than that in control group and T2 group (P<0.05), and the apparent metabolic rate of calcium (Ca) in all experimental groups was extremely higher than that in the control group (P<0.01). The ileal pH value of T3 group was significantly lower than that of other groups (P<0.05). Serum complement C3 content of broilers in each experimental group was significantly higher than that in the control group (P<0.05), and serum immunoglobulin G (IgG) and immunoglobulin M (IgM) content of broilers in T3 group were significantly higher than that in other groups (P<0.05). The villus height of T3 group was significantly higher than that of the control group and T1 group (P<0.05), and the villus-saphenous ratio of T3 group was significantly higher than that of the control group, T1 group, and T2 group (P<0.05). Subdoligranulum_variabile in T2 group and T3 group was significantly higher than that in control group and T1 group (P<0.05). Subdoligranulum_variabile in T2 group was extremely higher than that in the control group (P<0.01). The study indicates that the nutrient apparent metabolic rate, immune performance, intestinal structure, and intestinal flora were improved by adding ICGA in the diet. The appropriate level of ICGA in the diet of broilers is 2 000 mg/kg. [ABSTRACT FROM AUTHOR] |
