| Autor: |
Kuznetsova, Xenia, Dodueva, Irina, Afonin, Alexey, Gribchenko, Emma, Danilov, Lavrentii, Gancheva, Maria, Tvorogova, Varvara, Galynin, Nikita, Lutova, Lyudmila |
| Předmět: |
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| Zdroj: |
International Journal of Molecular Sciences; Jun2024, Vol. 25 Issue 11, p6236, 20p |
| Abstrakt: |
Spontaneous tumour formation in higher plants can occur in the absence of pathogen invasion, depending on the plant genotype. Spontaneous tumour formation on the taproots is consistently observed in certain inbred lines of radish (Raphanus sativus var. radicula Pers.). In this paper, using Oxford Nanopore and Illumina technologies, we have sequenced the genomes of two closely related radish inbred lines that differ in their ability to spontaneously form tumours. We identified a large number of single nucleotide variants (amino acid substitutions, insertions or deletions, SNVs) that are likely to be associated with the spontaneous tumour formation. Among the genes involved in the trait, we have identified those that regulate the cell cycle, meristem activity, gene expression, and metabolism and signalling of phytohormones. After identifying the SNVs, we performed Sanger sequencing of amplicons corresponding to SNV-containing regions to validate our results. We then checked for the presence of SNVs in other tumour lines of the radish genetic collection and found the ERF118 gene, which had the SNVs in the majority of tumour lines. Furthermore, we performed the identification of the CLAVATA3/ESR (CLE) and WUSCHEL (WOX) genes and, as a result, identified two unique radish CLE genes which probably encode proteins with multiple CLE domains. The results obtained provide a basis for investigating the mechanisms of plant tumour formation and also for future genetic and genomic studies of radish. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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