| Autor: |
Tripathi, Ayushi, Rathore, Meenal, Shukla, Susmita, Das, Alok, Debnath, Samir C. |
| Zdroj: |
Plant Cell, Tissue & Organ Culture; Jun2024, Vol. 157 Issue 3, p1-21, 21p |
| Abstrakt: |
Mungbean, a socioeconomically important legume, is a key player in the pursuit of food security and sustainable agriculture demanding attention for developing a highly efficient transformation system to support functional genomics and translational research. This study was focused to fill in the gap using both Agrobacterium-mediated and biolistic methods by experimenting with variables of the procedure to attain higher regeneration and transformation efficiencies. The study found comparable regeneration and transformation efficiencies between direct and indirect methods of gene transfer under selection pressure. Biolistic transformation of embryonic axis explants yielded an average transformation efficiency of 14.22% alongwith the one step regeneration method, whereas the Agrobacterium-mediated method involving a selection regime achieved an average efficiency of 1.19%. Presence and transmission of transgene across two generations were confirmed by PCR and Southern blot analyses in five mungbean lines. Two southern positive homozygous transgenic lines (T3 generation), when under PEG (Polyethylene glycol) induced drought stress, revealed AtDREB1a transgene expression and tolerance to the stress that was evaluated by morpho biochemical parameters including relative water content (RWC), membrane stability index (MSI), proline concentration and total chlorophyll content of the investigated lines. This study documents a first report of use of biolistic in mungbean supported by a one-step regeneration method that minimizes losses incurred during in vitro based regeneration of transformants and thus enhances the transformation efficiency. It also reports generation of transgenic mungbean in cultivar Samrat that is tolerant to PEG induced drought stress.Key message: We compared the biolistic and Agrobacterium-mediated modes of genetic transformation in mungbean highlighting the bottlenecks of the latter. AtDREB1a overexpression conferred drought stress tolerance to T3 transgenic lines. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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