| Abstrakt: |
Rauvolfia tetraphylla L. is a medicinal plant renowned for its pharmacological properties, faces threats due to habitat destruction and over-exploitation. This study targeted to explore micropropagation techniques to mitigate these challenges and investigated seed germination, callus formation from various explants, direct organogenesis, and somatic embryogenesis. Surface sterilization methods were optimized to enhance seed germination rates. The seed coat is hard it prevents the seed germination, to overcomethe seed dormancy and the seeds were treated with 4°C for 48 hours. The germination of 83.33% was achieved after the partial seed coat removal. Callus formation was induced from leaf, node, internode, and root explants using different hormone combinations. Maximum callus formation was observed on MS medium amended with 2,4-dichlorophenoxyacetic acid and 6 - benzyl amino-purine (2,4-D+BAP) in all explants. Hormone combinations also influenced direct root and shoot formation, with higher concentrations promoting greater responses. Somatic embryogenesis was induced in leaf and root explants using 2,4-D and BAP. Optimal concentrations (1.5 mg/L each) resulted in the formation of globular stage embryos. Histological analysis revealed distinct cellular processes during somatic embryogenesis, including embryo differentiation. It emphasizes the importance of hormone optimization and provides insights into the stomatic embryogenesis driving the plant regeneration in R. tetraphylla, offering valuable contributions to tissue culture protocols and conservation efforts for the endangered medicinal plant. [ABSTRACT FROM AUTHOR] |
