| Abstrakt: |
To gauge the degree of purity and recovery during the protein purification process, a method for calculating the total amount of protein in each phase is necessary. Particularly when measuring the total protein in cancer cells and the protein in human serum. As indicators of alterations in biochemical processes, it's critical to identify variations in cancer cell protein expression. Changes in specific enzymes in human serum act as biochemical change markers in a manner similar to functional proteins. Therefore, it is crucial to use a precise total protein measurement method. The amount of crude protein in biological materials, such as the protein content of cancer cells and human serum, can be measured using a number of reasonably quick, easy, and sensitive procedures. While some techniques may be effective at detecting soluble protein, it is necessary to show that they are appropriate for detecting protein in complex samples, such cell material. The Bradford and Biuret techniques are frequently employed. Both techniques rely on spectrophotometric measurements in the visible range and are based on the reaction of proteins with particular chemicals to create chrommopores. Both, however, have unique protein reaction targets and have some vulnerabilities against specific illnesses. Purpose: so this study aims to compare the total protein levels of colon cancer cells, lungs and human serum using both methods. Methode: The method used is the protein extraction of colon cancer cells and lung cancer with MPer solution, while the total protein measurement using the colorimetric method uses R biuret and R Bradford reagents which are read using an elisa reader. Findings: The total protein average Of Widr cell line, HTB 183 cell line and human plasma as follows, lowry methods (ug/ml) :1733,00± 65,05; 2110,50±71,42; 3733,00±176,77. Bradford methods (ug/ ml): 1439,00±120,52; 2045,67±194,71; 3930,67±314,91. Biuret method (ug/ml): 8834,20±703,05; 3598,53±147,56; 15671,53±1701,80. Implication: The Bradford and Lowry more sensitive and appropriate to test protein from cell line. The biuret more sensitive and appropriate to test protein from Human sample. [ABSTRACT FROM AUTHOR] |
