Central transcriptional regulator controls photosynthetic growth and carbon storage in response to high light.

Autor: Steichen, Seth, Deshpande, Arnav, Mosey, Megan, Loob, Jessica, Douchi, Damien, Knoshaug, Eric P., Brown, Stuart, Nielsen, Robert, Weissman, Joseph, Carrillo, L. Ruby, Laurens, Lieve M. L.
Předmět:
Zdroj: Nature Communications; 6/6/2024, Vol. 15 Issue 1, p1-14, 14p
Abstrakt: Carbon capture and biochemical storage are some of the primary drivers of photosynthetic yield and productivity. To elucidate the mechanisms governing carbon allocation, we designed a photosynthetic light response test system for genetic and metabolic carbon assimilation tracking, using microalgae as simplified plant models. The systems biology mapping of high light-responsive photophysiology and carbon utilization dynamics between two variants of the same Picochlorum celeri species, TG1 and TG2 elucidated metabolic bottlenecks and transport rates of intermediates using instationary 13C-fluxomics. Simultaneous global gene expression dynamics showed 73% of the annotated genes responding within one hour, elucidating a singular, diel-responsive transcription factor, closely related to the CCA1/LHY clock genes in plants, with significantly altered expression in TG2. Transgenic P. celeri TG1 cells expressing the TG2 CCA1/LHY gene, showed 15% increase in growth rates and 25% increase in storage carbohydrate content, supporting a coordinating regulatory function for a single transcription factor. Researchers identify unique transcriptional regulation that controls photosynthetic response, growth and biochemical carbon storage in high light for two variants of the same algae species, offering a glimpse into diel control of plant and crop yields. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
Externí odkaz: