A new green approach to l-histidine and β-alanine analysis in dietary supplements using rapid and simple contactless conductivity detection integrated with high-resolution glass-microchip electrophoresis.

Autor: Pukleš, Iva, Páger, Csilla, Sakač, Nikola, Matasović, Brunislav, Kovač-Andrić, Elvira, Šarkanj, Bojan, Samardžić, Mirela, Budetić, Mateja, Molnárová, Katarína, Marković, Dean, Vesinger, Ana, Jozanović, Marija
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Zdroj: Analytical & Bioanalytical Chemistry; Jun2024, Vol. 416 Issue 15, p3605-3617, 13p
Abstrakt: The analysis of dietary supplements is far less regulated than pharmaceuticals, leading to potential quality issues. Considering their positive effect, many athletes consume supplements containing l-histidine and β-alanine. A new microfluidic method for the determination of l-histidine and β-alanine in dietary supplement formulations has been developed. For the first time, capacitively coupled contactless conductivity detection was employed for the microchip electrophoresis of amino acids in real samples. A linear relationship between detector response and concentration was observed in the range of 10–100 µmol L–1 for l-histidine (R2 = 0.9968) and β-alanine (R2 = 0.9954), while achieved limits of detection (3 × S/N ratio) were 4.2 µmol L–1 and 5.2 µmol L–1, respectively. The accuracy of the method was confirmed using recovery experiments as well as CE-UV-VIS and HPLC-UV-VIS techniques. The developed method allows unambiguous identification of amino acids in native form without chemical derivatization and with the possibility of simultaneous analysis of amino acids with metal cations. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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