Potential use of dimethyl sulfoxide (DMSO) in artificial insemination of rabbits.

Autor: Fik, Martin, Kasarda, Radovan, Ondruška, L'ubomír, Kokoszyński, Dariusz, Lukáč, Norbert, Czerniawska-Piątkowska, Ewa, Biesiada-Drzazga, Barbara, Andraszek, Katarzyna, Banaszewska, Dorota
Předmět:
Zdroj: Animal Science & Genetics; 2024, Vol. 20 Issue 1, p23-32, 10p
Abstrakt: The aim of this work was to analyse the effect of incubation of a rabbit insemination dose (ID) with dimethyl sulfoxide (DMSO) on reproductive parameters (kindling rate, number of live-born and stillborn kits, and birth weight of kits). The experiments were carried out using two rabbit breeds. Each ejaculate was diluted to a concentration of 40 to 50 million sperm/0.5 ml ID. DMSO was added to the ID in a concentration of 0.5 M. The control ID did not contain any DMSO. Kindling rates obtained from two different breeds (P1, P2) showed minor improvement (+4.36% P1; +4.06% P2) compared to the controls, but without statistically significant differences. The number of live-born kits per litter obtained from breeds P1 and P2 showed minor improvement (P1 +0.61; P2 +0.13) compared to the control groups (p > 0.05). The weight of live-born kits was higher in the experimental groups (+3.85 g P1 = p = 0.001; +1.18 g P2 = p>0.05) than in the control groups. Monitoring of sperm quality indicators (1, 3 and 6 hours incubation) in in vitro conditions (CASA - Computer Assisted Semen Analysis) showed the best results in the control group that was not treated with DMSO. The data obtained in our experiments suggest positive effects of DMSO incubation on reproduction parameters, especially in vivo. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index