| Autor: |
Sieburth, Derek, QueeLim Ch'ng, Dybbs, Michael, Tavazoie, Masoud, Kennedy, Scott, Duo Wang, Dupuy, Denis, Rual, Jean-François, Hill, David E., Vidal, Marc, Ruvkun, Gary, Kaplan, Joshua M. |
| Předmět: |
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| Zdroj: |
Nature; 7/28/2005, Vol. 436 Issue 7050, p510-517, 8p |
| Abstrakt: |
Chemical synapses are complex structures that mediate rapid intercellular signalling in the nervous system. Proteomic studies suggest that several hundred proteins will be found at synaptic specializations. Here we describe a systematic screen to identify genes required for the function or development of Caenorhabditis elegans neuromuscular junctions. A total of 185 genes were identified in an RNA interference screen for decreased acetylcholine secretion; 132 of these genes had not previously been implicated in synaptic transmission. Functional profiles for these genes were determined by comparing secretion defects observed after RNA interference under a variety of conditions. Hierarchical clustering identified groups of functionally related genes, including those involved in the synaptic vesicle cycle, neuropeptide signalling and responsiveness to phorbol esters. Twenty-four genes encoded proteins that were localized to presynaptic specializations. Loss-of-function mutations in 12 genes caused defects in presynaptic structure. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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