| Autor: |
Felisberto, Mariano, Walter, Laura Otto, Cardoso, Chandra Chiappin, Santos-Pirath, Íris Mattos, Costa, Heloisa Zorzi, Gartner, Rafaela, Werle, Isabel, Mohr, Eduarda Talita Bramorski, Salvan da Rosa, Julia, Lubschinski, Tainá Larissa, Kretzer, Iara Fabricia, Masukawa, Ivete Ioshiko, de Almeida Vanny, Patrícia, Luiz, Magali Chaves, Rabello de Moraes, Ana Carolina, Santos-Silva, Maria Claudia, Dalmarco, Eduardo Monguilhott |
| Předmět: |
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| Zdroj: |
Journal of Applied Laboratory Medicine; May2024, Vol. 9 Issue 3, p456-467, 12p |
| Abstrakt: |
Background: In view of the scientific gap in knowledge of the involvement of the B-cell compartment and clinical prognostic in SARS-CoV-2 infection, this work aims to evaluate the B-cell subsets and the presence of specific IgM and IgG, as well as neutralizing antibodies against SARS-CoV-2, in unvaccinated patients diagnosed with COVID-19. Methods: This study included 133 patients with COVID-19. Cellular components were assessed by flow cytometry, and immunoglobulin levels and reactivity were measured by indirect enzyme-linked immunosorbent assay. Results: Our results showed no changes in less differentiated B cells. However, non-switched memory B cells (NS-MBCs) and class-switched memory B cells (CS-MBCs) were reduced in the patients with moderate disease. Also, plasmablasts and double-negative (DN) or "atypical" memory B cells were increased in groups of patients with moderate to critical conditions. In addition, the production of IgM, IgG, and neutralizing antibodies against SARS-CoV-2 demonstrated a positive correlation between the positivity of antibodies against SARS-CoV-2 and disease severity. Besides being related to the development of a more severe course of the disease, the increase in DN B-cell count also contributed to a poorer disease outcome in patients with a higher percentage of these cells. On the other hand, we observed an increase in the absolute number of CS-MBCs in patients with greater chances of survival. Conclusions: This study demonstrates that the B-cell compartment may contribute to the development of clinical symptoms of COVID-19, with changes in B-cell subset counts linked to disease course and patient prognosis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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