Autor: |
Lor, Yer, Kaminski, Marissa, Spear, Stephen, Klymus, Katy, Richter, Cathy, Kroboth, Patrick, Chapman, Duane, Merkes, Christopher, Thompson, Nathan |
Předmět: |
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Zdroj: |
Annual Conference on Great Lakes Research Abstract Book; 2023, Vol. 66, p122-123, 2p |
Abstrakt: |
Invasive species can be detrimental to the environments they invade and outcompete native species. Increased captures of wild Black carp (Mylopharyngodon piceus), an invasive molluscivore, have raised concerns of these species impacting native imperiled unionid mussel populations. Poulton et al. (2019) examined the digestive contents of over 100 fish captured from lentic and lotic habitats, but visual identification can be extremely challenging. The use of genetic identification and metabarcoding may allow for a more efficient and consistent way to identify Black carp diets. We use DNA metabarcoding to identify diet contents of wild-caught Black carp and compare them with the visual identification of the diets. We extracted DNA from gut contents of 58 wild-caught black carp preserved in ethanol and used two unionid metabarcoding assays that target COI and ND1 genes to identify unionid mussel species. We also used a general macroinvertebrate marker to identify other prey species consumed by Black carp. We will present the comparison between prey species identified with metabarcoding and visual identification. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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