| Autor: |
Guo, Mengjiao, Jin, Yikun, Wang, Haonan, Zhang, Xiaorong, Wu, Yantao |
| Předmět: |
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| Zdroj: |
Veterinary Sciences; May2024, Vol. 11 Issue 5, p208, 11p |
| Abstrakt: |
Simple Summary: Infectious coryza is an acute respiratory infectious disease of chickens caused by Av. paragallinarum. Av. paragallinarum can be serotyped into A (A1–A4), B (B-1), and C (C1–C4) serovars. However, there is no effective molecular typing scheme to gain basic knowledge about the Av. paragallinarum population structure. MLST has played a major role in investigating the genetic structure of bacterial populations and has rapidly become the cornerstone technique for the molecular typing of pathogenic microorganisms. In this study, we are the first to establish this method and applied to PubMLST for a public MLST site for Av. paragallinarum. More samples and the ST sequence data can help to clearly observe the genetic evolution and epidemic patterns of Av. paragallinarum. Infectious coryza is an acute respiratory infection caused by Avibacterium paragallinarum, which is widely distributed throughout the world. However, there is no effective molecular typing scheme to obtain basic knowledge about the Av. paragallinarum population structure. This study aimed to develop a multilocus sequence typing (MLST) scheme for Av. paragallinarum that allows for the worldwide comparison of sequence data. For this purpose, the genetic variability of 59 Av. paragallinarum strains from different geographical origins and serovars was analyzed to identify correlations. The MLST scheme was developed using seven conserved housekeeping genes, which identified eight STs that clustered all of the strains into three evolutionary branches. The analytical evaluation of the clone group relationship between the STs revealed two clone complexes (CC1 and CC2) and three singletons (ST2, ST5, and ST6). Most of the isolates from China belonged to ST1 and ST3 in CC1. ST8 from Peru and ST7 from North America together formed CC2. Our results showed that the Av. paragallinarum strains isolated from China had a distant genetic relationship with CC2, indicating strong regional specificity. The MLST scheme established in this study can monitor the dynamics and genetic differences of Av. paragallinarum transmission. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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