| Abstrakt: |
Introduction The routine testing and monitoring includes the parameters such as FBS, PBBS, HbA1C. Currently the vaccutainers or collection tubes for FBS, PBBS, RBS contains Grey capped sodium Fluoride, which inhibits glycolysis there by preventing glucose from utilization on long standing, and HbA1C is being collected in EDTA containing vacutainer with purple cap. Very few studies have done a comparison of HbA1C values by High performance liquid chromatography method using the sample collected in fluoride Grey cap and EDTA purple cap vaccutainers. The Study aims to compare locally made vaccutainers for the analysis of HbA1C. Materials and Methods: Blood samples were collected in EDTA and Sodium fluoride vacutainers were analyzed in D10 HPLC auto analyzer. Results: There was no significant variation in HbA1C levels by using both EDTA and Sodium Fluoride vacutainers in study group with low, normal & high levels of HbA1C in all study group participants by using same Sodium Fluoride vacutainer. So same vacutainer can be used for both blood Sugar & HbA1C estimations, to decrease the cost burden, turnaround time, manpower, and sample quantity to patient. Conclusions: HbA1C assay is regularly done by using EDTA vacutainer as per manufacturer's recommendations. Present study proved that Glycated HbA1c assay can also be done by using Sodium fluoride vacutainer, which is used for estimation of FBS, PLBS & RBS, so, a single vacutainer can be used for both Blood Sugar and HbA1C estimations. [ABSTRACT FROM AUTHOR] |
