| Abstrakt: |
In the present study, three purple non sulphur bacterial strains were isolated from water and soil samples and were characterised. The three bacterial strains were identified as Rhodopsuedomonas palustris RM01, Rhodopsuedomonas rhenobacensis RM02 and Rhodopsuedomonas palustris RM03 strains. All the three Rhodopseudomonas species strains were nonmotile and the absorption spectral characters revealed the presence of bacteriochlorophyll "a" by ultra-visible spectroscopy. Rhodopseudomonas palustris RM01 showed an absorption maxima at 673, 806 and 865 nm) and the type of carotenoids as spirilloxanthin (absorption maxima at 389, 474, 502, 592 and 530 nm), Rhodopseudomonas rhenobacensis RM02 absorption maxima have seen at 806 and 867 nm and the type of carotenoids as spirilloxanthin (absorption maxima at 382, 473, 503, 591 and 530 nm) and Rhodopseudomonas palustris RM03 absorption maxima was at 759, 805 and 874 nm) and the type of carotenoids as spirilloxanthin (absorption maxima at 376, 492 and 591 nm). RM01, RM02, and RM03 strains were shown a dry biomass yield was 12.1grams, 16.8grams and 13.6grams dry weight per litre, respectively. All the isolated PNSB strains could grow in a range of 6-7 pH tested. Sequence data of the strain was deposited in NCBI (National Centre for Biotechnology Information). These three bacteria Rhodopseudomonas palustris RM01, Rhodopseudomonas. Rhenobacensis RM02 and Rhodopseudomonas palustris RM03 have optimal growth at 28°C & 30°C respectively. Further morphological characterization of these three strains were carried out on the basis of microscopic observations, SEM studies, colour of cell suspensions, absorption spectral characters, organic and inorganic substrate utilization, vitamin requirement, temperature growth and biomass production and 16S rRNA gene sequencing analysis. They were identified and named as Rhodopseudomonas palustris RM01, Rhodopseudomonas rhenobacensis RM02 and Rhodopseudomonas palustris RM03. These were further screened for biomass production. [ABSTRACT FROM AUTHOR] |
