| Autor: |
Rajagopalan, Gobinath, Jain, Akanksha, Poosarla, Venkata Giridhar, Krishnan, Chandraraj, Yang, Kun Lin |
| Předmět: |
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| Zdroj: |
Journal of Chemical Technology & Biotechnology; Jun2024, Vol. 99 Issue 6, p1445-1458, 14p |
| Abstrakt: |
BACKGROUND: 1,3‐Propanediol (PDO) is a raw material/specialty chemical/additive in several industrial applications. A new wild‐type Clostridium strain BOH3 can utilize crude glycerol (CG) and produce PDO. This investigation reveals its potential for PDO production from CG. RESULTS: Initially, inoculum of strain BOH3 was cultured from reinforced clostridial medium (RCM) with glucose (medium‐A), and modified‐RCM with glycerol (medium‐B). While fermenting 20 g L−1 glycerol, the cells cultivated from medium‐A produced 5.75 g L−1 PDO (as major product) with 3.10 g L−1 butanol (byproduct), whereas the cells cultivated from medium‐B produced 11.01 g L−1 PDO with 2.50 g L−1 butyric acid and 1.01 g L−1 acetic acid, with no butanol produced. To enhance PDO production from the batch‐process, various parameters including pure glycerol/CG concentration (80 g L−1), inoculum age (15 h) and size (12.50%v/v) of cells from medium‐B, initial medium‐pH (pH 6.4) and incubation temperature (39 °C) were studied; the optimal values obtained are given in their respective parenthesis. Under optimal conditions, PDO production was 42.58–47.15 g L−1 (0.65–0.79 mol‐PDO/mol‐glycerol) from CG‐based rich/mineral media. Furthermore, fed‐batch fermentation with 50–80 g L−1 initial CG‐concentration was tested to enhance the PDO production. Among these, 60 g L−1 initial CG‐concentration assisted strain BOH3 in utilizing a high level of CG (~174 g L−1); it supported a higher‐titer of PDO production (103.55–116.45 g L−1), with the highest yield (0.72–0.81 mol−PDO/mol−glycerol) ever reported. CONCLUSION: Clostridium strain BOH3 shows the potential to support a high titer (>116 g L−1) with the highest yield (>0.80 mol mol−1) of PDO production from CG (a waste). It can be considered for industrial‐scale PDO production. © 2024 Society of Chemical Industry (SCI). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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