| Autor: |
Lin, Cui-Lian, Chen, Wen-Da, Liu, Li, Cheng, Liang |
| Předmět: |
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| Zdroj: |
Chemical Communications; 5/14/2024, Vol. 60 Issue 39, p5197-5200, 4p |
| Abstrakt: |
Through the integration of CRISPR/Cpf1 with optogenetics and a reduction-responsive motif, we have developed a photoactivatable cross-linked crRNA that enables precise genome editing upon light exposure. This system also allows for termination of editing activity through external application of reducing agent. The dual-stimuli-responsive CRISPR/Cpf1 editing process operates in a unique OFF → ON → OFF sequence, making it a valuable tool for investigating time-sensitive biological events. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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