| Abstrakt: |
The role of this study was to evaluate the impact of gut microbiota depletion on the progression of osteoarthritis (QA) and osteoporosis (QP). We conducted an experimental mouse model of QA and QP over an 8-week period. The model involved destabilization of the medial meniscus and bilateral ovariectomy (QVX). To deplete the gut microbiota, we administered a course of antibiotics for 8 weeks. The severity of QA was assessed through micro-CT scanning, X-rays, and immunohistochemical staining. Microbiome analysis was performed using PCR of 16S DNA on fecal samples, and the levels of serum lipopolysaccharide, interleukin 6, tumor necrosis factor-α (TNF-α), osteocalcin, and estrogen were measured using enzyme-linked immunosorbent assay. We found that in comparison to the QVX+QA group, the QVX+QA+ABT group exhibited increased bone mineral density (P < 0.0001), bone volume fraction (P = 0.0051), and trabecular number (P = 0.0023) in the metaphyseal bone. Additionally, cartilage injury and levels of matrix metalloproteinase 13 were reduced in the QVX+QA+ABT group compared to the QVX+QA group. Moreover the QVX+QA+ABT group demonstrated decreased relative abundance of Bacteroidetes, serum lipopolysaccharide (P = 0.0005), TNF-α (P < 0.0001), CTX-1 (P = 0.0002), and increased expression of bone formation markers. These findings were further supported by correlation network analyses. Depletion of gut microbiota was shown to protect against bone loss and cartilage degradation by modulating the composition of the gut microbiota in osteoporosis and osteoarthritis. [ABSTRACT FROM AUTHOR] |
