| Abstrakt: |
Objective: To observe the effect and possible mechanism of action of Bushen Bitong recipe (BSBT) containing serum on IL-1β-induced chondrocyte apoptosis. Methods: Generation 3 rat chondrocytes were randomized into Control, IL-1β, IL-1β+BSBT (L), IL-1β+BSBT (M), and IL-1β+BSBT (H) groups (5%, 10% and 15% BSBT-containing serum), and then 24h after intervention respectively, the cell proliferation and Apoptosis rate; Western blot detected the expression levels of Bcl-2, BAX, Caspase-3, SOX9, NF-κB p65, MMP-13 proteins in chondrocytes. ELISA detected the levels of TNF-α, IL-6, and bFGF in the supernatants of chondrocyte culture. Results: Compared with Control group, cell proliferation activity decreased, apoptosis rate increased, NF-κB p65, MMP-13 protein level and TNF-α, IL-6 level increased, and SOX9 protein level and bFGF level decreased in IL-1β group; compared with IL-1β group, different concentrations of BSBT-containing serum group, cell proliferation activity increased, and apoptosis rate decreased. NF-κB p65, MMP-13 protein level and TNF-α, IL-6 level decreased, SOX9 protein level and bFGF level increased; compared with IL-1β+BSBT (L) group, cell proliferation activity increased, apoptosis rate decreased in IL1β+BSBT (M) and IL-1β+BSBT (H) groups, and NF-κB p65, MMP-13 protein level and TNF-α level decreased. 13 protein levels and TNF-α and IL-6 levels decreased, and SOX9 protein levels and bFGF levels increased. Conclusion: BSBT-containing serum may promote IL-1β-induced proliferation of chondrocytes, reduce apoptosis, improve the microenvironment of chondrocytes, and promote cartilage repair through the SOX9/NF-κB/MMP-13 signaling pathway. [ABSTRACT FROM AUTHOR] |
