| Autor: |
B. Teresa, Maira Theresa, Trajano, Patricia Irhene V., M. Dela Rosa, Angelo Mc Court, Dimayacyac, Airianne Denise C., Ipulan-Colet, Lerrie Ann D. G. |
| Předmět: |
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| Zdroj: |
Philippine Journal of Science; Feb2024, Vol. 153 Issue 1, p47-60, 14p |
| Abstrakt: |
Incomplete muscle regeneration after severe injury can cause the formation of fibrotic tissue, which can impair muscle function and decrease quality of life. Myogenic cell culture studies have been utilized to analyze the effects of certain compounds on muscle cells. Garcinia binucao crude leaf extracts (GB) were tested on C2C12 cells to determine their effect on myoblast count and myotube formation in a dose-dependent manner. For myoblast count, C2C12 myoblasts were observed after 24 and 48 h of GB treatment. Based on the results, GB extracts did not confer cytotoxic effects, and precocious myotube formation was observed after 48 h of treatment. Ki67 expression was not statistically significant among treatment groups. For induction of differentiation assay, the myotube area - stained with May-Grünwald Giemsa - was examined at 1, 3, and 7 d post-treatment (dpt) and images were obtained and analyzed using ImageJ. For induction assay at 3 dpt, a higher myotube area was observed as the concentration of the extracts decreased. Concentrations of 0.781, 0.391, and 0.195 µg/mL showed significant differences compared to growth medium control when observed at 3 dpt (p < 0.05). There were also no observed significant differences in MyoD expression, a myoblast marker. The increased size of the myotube may be explained based on glucose consumption, which was examined by acquiring glucometer readings from spent cell culture media. The glucometer reading shows a statistically significant lower glucometer reading in the GB-treated group. These indicate that the extract may contain compounds that promote myoblast growth and increase myotube size. Phytochemical analysis revealed the presence of specific secondary metabolites, which may contribute to the effect observed in this study. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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