| Autor: |
Armando, Federico, Porcellato, Ilaria, de Paolis, Livia, Mecocci, Samanta, Passeri, Benedetta, Ciurkiewicz, Małgorzata, Mechelli, Luca, Grazia De Ciucis, Chiara, Pezzolato, Marzia, Fruscione, Floriana, Brachelente, Chiara, Montemurro, Vittoria, Cappelli, Katia, Puff, Christina, Baumgärtner, Wolfgang, Ghelardi, Alessandro, Razzuoli, Elisabetta |
| Předmět: |
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| Zdroj: |
Veterinary Pathology; May2024, Vol. 61 Issue 3, p366-381, 16p |
| Abstrakt: |
Vulvo-vaginal epithelial tumors are uncommon in mares, and data on the epithelial-to-mesenchymal transition (EMT) and the tumor-immune microenvironment (TIME) are still lacking. This is a study investigating the equus caballus papillomavirus type 2 (EcPV2) infection state as well as the EMT process and the tumor microenvironment in vulvo-vaginal preneoplastic/ benign (8/22) or malignant (14/22) epithelial lesions in mares. To do this, histopathological, immunohistochemical, transcriptomic, in situ hybridization, and correlation analyses were carried out. Immunohistochemistry quantification showed that cytoplasmic E-cadherin and β-catenin expression as well as nuclear β-catenin expression were features of malignant lesions, while benign/preneoplastic lesions were mainly characterized by membranous E-cadherin and β-catenin expression. Despite this, there were no differences between benign and malignant equine vulvo-vaginal lesions in the expression of downstream genes involved in the canonical and noncanonical wnt/β-catenin pathways. In addition, malignant lesions were characterized by a lower number of cells with cytoplasmic cytokeratin expression as well as a slightly higher cytoplasmic vimentin immunolabeling. The TIME of malignant lesions was characterized by more numerous CD204+ M2-polarized macrophages. Altogether, our results support the hypothesis that some actors in TIME such as CD204+ M2-polarized macrophages may favor the EMT process in equine vulvo-vaginal malignant lesions providing new insights for future investigations in the field of equine EcPV2-induced genital neoplastic lesions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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