| Abstrakt: |
Metformin extract is distinguished by its therapeutic ability for many diseases and microbial revival, which increases the antioxidant capacity, inhibits inflammatory processes, and improves the metabolism of fats and blood sugar. The therapeutic efficacy of plant extracts is very important and has complementary efficacy, for example, Viscum L (VA) plant extract is frequently used in integrative medicine to reduce side effects in cancer patients, especially in ovarian cancer patients. The number of ovarian cancer patients in Iraq is increasing. The purpose of this study was to investigate the therapeutic effect of Viscum extract, metformin, on ovarian cancer cells. Ovarian cancer cell (Ovcar-3) line was treated with metformin, Viscum album (mistletoe) seeds extract, laser irradiation, Viscum album (mistletoe) seeds with lased irradiation, and metformin with laser irradiation. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide cytotoxicity (MTT) assay was employed to evaluate the cellular effect of these treatments. The results showed that the viability of Ovcar-3 cells was significantly decreased with increasing Viscum album seeds extract concentration. Green Laser at 532 nm and 286 mW considerably reduced cells viability within relatively short exposure times. Cells growth inhibition of 25% was achieved after five minutes of laser irradiation. Treatments of the cells with different concentrations of plant extract followed by laser irradiation intensely reduced the cell viability to less than 15% compared to the control group. Treating the cells with metformin significantly decreased cell growth and proliferation, particularly at the metformin concentration of 31.25 µg/mL. Furthermore, a combination of metformin and laser irradiation decreased the cells viability by 125%. The results showed that Viscum album seeds extract and metformin had distinct effects in reducing the proliferation and vitality of cancer cells significantly, which might reach the therapeutic effectiveness of chemotherapy and radiation safer and with less side effects. When these two extracts combined with green laser light, the effectiveness in inhibiting the growth of cancer cells increased greatly. [ABSTRACT FROM AUTHOR] |
