| Abstrakt: |
A water-soluble curcuminoid powder was developed using cyclodextrins and quantified individual curcuminoids using high-performance liquid chromatography (HPLC). The standard 95% curcuminoid powder (CSTD) employed in our study consists of demethoxycurcumin (DMC, 19.29%), bis-demethoxy curcumin (BDMC, 4.60%) and curcumin (71.11%). In water-soluble products, we observed that all three curcuminoids were present in equal proportions, i.e. 1:1:1 ratio and its solubility was found to be 40,000 ppm, while the curcumin powder solubility is about 5 ppm.Water-soluble curcuminoid powder was obtained by the encapsulation method using cyclodextrins namely, beta-cyclodextrin (BCD) and beta-methyl cyclodextrin (BMCD). In the encapsulation method, compounds are embedded inside lipophilic cores of encapsulating agents without any change in chemical structure. Analogous to micelles, encapsulating agents have hydrophilic groups lying outside the core and when they come in contact with water they get dissolved by the release of curcuminoids. In-vitro antioxidant properties assessed by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method showed IC50 values for water-soluble curcuminoids at 10.4 ± 0.18 µg/ml for WSC-1, 30.3 ± 1.16 µg/ml for WSC-2 and 18.73 ± 0.58 µg/ml for WSC-3, while for the standard curcuminoid (CSTD) and ascorbic acid values of 27.56 ± 0.06 µg/ml and 45.69 ± 0.66 µg/ml, respectively, were found. Cell line studies using RAW 264.7 revealed that our encapsulated water-soluble powders, viz. WSC-1 (with BMCD alone), WSC-2 (with BCD alone), WSC-3 (with BCD and BMCD 1:1), showed IC50 values of cell line studies 1.33 ± 0.19, 27.43 ± 0.04 and 1.27 ± 0.02 µg/ml when compared to CSTD which was 38.45 ± 0.28 µg/ml (p < 0.05). These positive initial leads on cell line studies of these water-soluble curcuminoids helped to focus on the anti-inflammatory and anti-cancer activity on solid tumors. [ABSTRACT FROM AUTHOR] |
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